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4.2 The ``Single force'' option

This option is used to force a group of consecutive bases to be double-stranded. After this item is selected from the main menu, the user is prompted by: ``Enter base and length''. The historical 5′ base number of the segment, i, should be entered, followed by the number of bases that are to be double-stranded, k. Thus bases r_i ,r_i+1 , ... ,r_i+k-1 are forced to be double-stranded. Caution should be exercised when using this option.

Michael Zuker
Thu Nov 2 14:28:14 CST 1995