RNAfolding Error
Posted: Thu Aug 25, 2022 8:57 pm
Hello,
I have been experimenting with mFold and have come across an odd feature and was looking for clarification. When I fold a 140bp segment of RNA nucleotides, it appears fine. The mFold server outputs 4 possible RNA structures that I can view once I download them. These are available to download under "Download all foldings" subheader. However, I am interested in the difference in outputs following SNP mutations. Sometimes, when I change 1-3nts in the 140bp RNA, it will still allow me to download possible structures, but sometimes, it completely removes the option and the only subheader is "Output". I can no longer view Download all foldings, view ss-count information, view individual structures.
I was hoping to get some clarification as to why this is happening? Is this the software saying there were no folds it could reasonably come up with?
Any help would be appreciated and thanks in advance,
Virology Candidate
I have been experimenting with mFold and have come across an odd feature and was looking for clarification. When I fold a 140bp segment of RNA nucleotides, it appears fine. The mFold server outputs 4 possible RNA structures that I can view once I download them. These are available to download under "Download all foldings" subheader. However, I am interested in the difference in outputs following SNP mutations. Sometimes, when I change 1-3nts in the 140bp RNA, it will still allow me to download possible structures, but sometimes, it completely removes the option and the only subheader is "Output". I can no longer view Download all foldings, view ss-count information, view individual structures.
I was hoping to get some clarification as to why this is happening? Is this the software saying there were no folds it could reasonably come up with?
Any help would be appreciated and thanks in advance,
Virology Candidate