UNAFold

Hello, How will I know if my DNA sequence is linear or circular. Below are my DNA Sequences of Aptamers.

1. 5’-SH-(CH2)6-GGCGCCAAACAGGACCACCATGACAATTACCCATACCACCTCATTATGCCCCATCTCCGC-3’

2. 5’-NH2-(CH2)6-ATAAGAGCAAAATGCGGCAGAACAACGCAAACGGCTGTCATGGTGGTCCTGTTTGGCGCC-(CH2)6-SH-3’

3.5’-NH2-(CH2)6-ATAAGAGCAAAATGCGGCAGGGTGGTATGGGTAATTGTCA-N20-TGGTGGTCCTGTTTGGCGCC-(CH2)6-SH-3’

4.5’-NH2-(CH2)6-GCGGAGATGGGGCATAATAGAACAACGCAAACGGCCACTGCAGTGGTCCTGTTTGGCGCC-(CH2)6-SH-3’

5.5’-SH-(CH2)6-CGTACGGTTAACGTTCGGCGTA CACTTCAA CTACGGAAT-3’

The fact that you've written them all with 5' at one end and 3' at the other suggests to me that they're linear. However, they're your sequences, not mine so only you know for sure!