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   <h2 class="title">Job Parameters</h2>
   <p>Submitted at Mon 20 Apr 2026 05:58:35 AM UTC</p>
   <p><code>5'-TGTTGTACTAGTGCCAACTGCTGATGGAGGA-3'</code> vs. <code>5'-ACCTCCGTCAGCAGTTGGCACAACTACAAGA-3'</code><br />
   From 0&deg;C by 1&deg;C to 95&deg;C as DNA<br />
   [A<sub>0</sub>] = 2e-08 M, [B<sub>0</sub>] = 2e-08 M<br />
   [Na<sup>+</sup>] = 0.05 M, [Mg<sup>++</sup>] = 0.003 M (oligo mode)
   </p>
   <p>Computation took 1:44 (<a href="log.txt">Log file</a>)</p>

   <h2>Plots</h2>
   <table>
    <tr><th colspan="2"><a href="https://www.unafold.org/help-output.php#conc">Concentration Plot</a></th></tr>
    <tr><td colspan="2"><a href="javascript:void(window.open('conc-big.png', '', 'width=740,height=524'))"><img src="conc.png" width="320" height="224" alt="Concentration plot" /></a></td></tr>
    <tr><td colspan="2">Download: <a href="A-B.conc.ps" title="Concentration plot (Postscript)">Postscript</a> <a href="A-B.conc.pdf" title="Concentration plot (PDF)">PDF</a> <a href="A-B.conc" title="Concentration plot (text)">Text</a></td></tr>
    <tr>
     <th><a href="https://www.unafold.org/help-output.php#Cp">Heat Capacity Plot</a></th>

     <th><a href="https://www.unafold.org/help-output.php#ext">Absorbance Plot</a></th>

    </tr>
    <tr>
     <td><a href="javascript:void(window.open('Cp-big.png', '', 'width=740,height=524'))"><img src="Cp.png" width="320" height="224" alt="Heat capacity plot" /></a></td>

     <td><a href="javascript:void(window.open('ext-big.png', '', 'width=740,height=524'))"><img src="ext.png" width="320" height="224" alt="Extinction plot" /></a></td>

    </tr>
    <tr align="center">
     <td>Download: <a href="A-B.Cp.ps" title="Heat capacity plot (Postscript)">Postscript</a> <a href="A-B.Cp.pdf" title="Heat capacity plot (PDF)">PDF</a> <a href="A-B.ens.Cp" title="Heat capacity plot (text)">Text</a></td>

     <td>Download: <a href="A-B.ext.ps" title="Absorbance plot (Postscript)">Postscript</a> <a href="A-B.ext.pdf" title="Absorbance plot (PDF)">PDF</a> <a href="A-B.ens.ext" title="Asorbance plot (text)">Text</a></td>

    </tr>
    <tr align="center">
     <td>Download detailed plot: <a href="A-B.Cp2.ps" title="Detailed heat capacity plot (Postscript)">Postscript</a> <a href="A-B.Cp2.pdf" title="Detailed heat capacity plot (PDF)">PDF</a></td>

     <td>Download detailed plot: <a href="A-B.ext2.ps" title="Detailed absorbance plot (Postscript)">Postscript</a> <a href="A-B.ext2.pdf" title="Detailed absorbance plot (PDF)">PDF</a></td>

    </tr>
   </table>

   <h2>Probability Dot Plots</h2>
   <form action="https://www.unafold.org/cgi-bin/DINAMelt/hybrid2-plot.cgi" target="pic" method="get" onsubmit="window.open('', 'pic', 'width=632,height=632')"><p>
    <input type="hidden" name="tag" value="260420/055634" />
    <input type="submit" value="View" /> <label for="species">plot of</label>
    <select id="species" name="species">

     <option value="A">A</option>

     <option value="B">B</option>

     <option value="A-A">A-A</option>

     <option value="B-B">B-B</option>

     <option value="A-B">A-B</option>

    </select> <label for="temperature">at</label>
    <select id="temperature" name="temperature">

     <option value="0">0</option>

     <option value="1">1</option>

     <option value="2">2</option>

     <option value="3">3</option>

     <option value="4">4</option>

     <option value="5">5</option>

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     <option value="19">19</option>

     <option value="20">20</option>

     <option value="21">21</option>

     <option value="22">22</option>

     <option value="23">23</option>

     <option value="24">24</option>

     <option value="25">25</option>

     <option value="26">26</option>

     <option value="27">27</option>

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     <option value="29">29</option>

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     <option value="50">50</option>

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     <option value="53">53</option>

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     <option value="92">92</option>

     <option value="93">93</option>

     <option value="94">94</option>

     <option value="95">95</option>

    </select> &deg;C
   </p></form>

   <h2>Van't Hoff Plots</h2>
   <form action="https://www.unafold.org/cgi-bin/DINAMelt/hybrid2-vHp.cgi" method="post"><p>
    <input type="hidden" name="tag" value="260420/055634" />
    <input type="hidden" name="NA" value="DNA" />
    <input type="hidden" name="Ct" value="4e-08" />
    <input type="hidden" name="exclude" value="" />
    <input type="hidden" name="fraction" value="0.1" />
    <input type="hidden" name="Tmelt" value="50" />
    View a van't Hoff plot as... <input type="submit" name="format" value="PNG" /> <input type="submit" name="format" value="Postscript" /> <input type="submit" name="format" value="PDF" /> <input type="submit" name="format" value="Text" /><br />
    The fist time you request a plot there will be a short delay while the van't Hoff data is calculated.
   </p></form>



   <h2>Isothermal Titration Calorimetry Plots</h2>
   <form action="https://www.unafold.org/cgi-bin/DINAMelt/hybrid2-ITC.cgi" method="post"><p>
    <input type="hidden" name="tag" value="260420/055634" />
    <input type="hidden" name="NA" value="DNA" />
    <input type="hidden" name="A0" value="2e-08" />
    <input type="hidden" name="B0" value="2e-08" />
    <input type="hidden" name="exclude" value="" />
    <input type="hidden" name="fraction" value="0.1" />
    <input type="hidden" name="Tmelt" value="50" />
    <label for="vary">Vary concentration of</label> <select id="vary" name="vary"><option value="A">A</option><option value="B">B</option></select><br />
    <label for="temp">Enter one or more temperatures, separated by commas</label>: <input type="text" id="temp" name="temp" value="" size="10"/><br />
    View ITC plot as... <input type="submit" name="format" value="PNG" /> <input type="submit" name="format" value="Postscript" /> <input type="submit" name="format" value="PDF" /> <input type="submit" name="format" value="Text" /><br />
    There will be a short delay while the ITC data is calculated.
   </p></form>

   <h2>Important Numbers</h2>
   <p>
    <a href="https://www.unafold.org/help-output.php#TmConc">T<sub>m</sub>(Conc)</a>: 72.7&deg;C<br />
    <a href="https://www.unafold.org/help-output.php#dG">&#916;G</a>: 38.2 kcal/mol &nbsp; <a href="https://www.unafold.org/help-output.php#dH">&#916;H</a>: 210.9 kcal/mol &nbsp; <a href="https://www.unafold.org/help-output.php#dH">&#916;S</a>: 638.5 cal/mol/K &nbsp; <a href="https://www.unafold.org/help-output.php#TmCp">T<sub>m</sub>(C<sub>p</sub>)</a>: 59.8&deg;C
<br />
    <a href="https://www.unafold.org/help-output.php#TmExt1">T<sub>m</sub>(Ext1)</a>: 58.1&deg;C
    &nbsp;
    <a href="https://www.unafold.org/help-output.php#TmExt2">T<sub>m</sub>(Ext2)</a>: 57.7&deg;C

   </p>
   <p>Download the entire job with all files, formatted as:
    <a href="https://www.unafold.org/cgi-bin/DINAMelt/hybrid2-archive.cgi?tag=260420/055634&amp;format=bz2">tar/bzip2</a>
    <a href="https://www.unafold.org/cgi-bin/DINAMelt/hybrid2-archive.cgi?tag=260420/055634&amp;format=gz">tar/gzip</a>
    <a href="https://www.unafold.org/cgi-bin/DINAMelt/hybrid2-archive.cgi?tag=260420/055634&amp;format=zip">zip</a>
    <br />The first time you ask for an archive there may be a slight delay while it is created.
   </p>

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