Ensemble Calc

Reference: Quantitative prediction of miRNA-mRNA interaction based on equilibrium concentrations
Authors: Chikako Ragan, Michael Zuker & Mark A. Ragan
Published: PLoS Computational Biology (In press 2011)

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Web Application

• Enter the hybridization free energy of the oligo to the target:    
• Enter the free energy of the folded oligo:       (This is unually 0 since a short oligo rarely folds.)
• Enter the free energy of the folded target:    
• Enter the free energy of the folded target with the hybridization site constrained to be single-stranded:    
• Enter the total strand concentration of the oligo:       (Default: 1000 nM = 1 μM)
• Enter the total strand concentration of the target:       (Default: As above.)
• Select concentration units nM μM mM
• Enter the temperature deg C: