As-sgRNA is the 493735th nucleic acid sequence folded on the Amazon hosted mfold server. - Tuesday, April 21 06:45:13 UTC 2026 -

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Folding As-sgRNA at 37° C. (2.3)
- Computed for 111.43.19.16 -

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Linear RNA folding at 5%, window = 3, max folds = 50 51 A's, 36 C's, 50 G's, 37 U/T's and 0 N's. 10 20 30 40 50 GGGAUUCGUC GGUUCAGCGA CGAUAAGCCG AGAAGUGCCA AUAAAACUGU 60 70 80 90 100 UAAGUGGUUU GGUAACGCUC GGUAAGGUAG CCAAAAGGCU GAAACUCCGU 110 120 130 140 150 GCACAAAGAC CGCACGGACG CUUCACAUAU AGCUCAUAAA CAAGGGUUUG 160 170 180 CGAGCUAGCU UGUGGAGUGU GAAC

Output

The energy dot plot for As-sgRNA. (Definition)
File formats: Text, PostScript, pdf, png, jpg

Computed Structure:   (File Formats)
The computed folding contains 55 base pairs out of 75 (73.3%) in the energy dot plot.

Extra files:   sorted ct file;   h-num values;   p-num values;   log file for main computations.

Circular structure Plots

◈ Structure 1 : ΔG = -68.80 kcal/mol, (Thermodynamic Details).
Different file formats: PostScript, pdf, png, jpg, .ct file, Vienna, RNAML, RnaViz ct, Mac ct, RNAdraw, XRNA ss.

These computations were performed on ip-172-31-2-102 in 0 min. and 13.75 sec. and can be accessed at http://www.unafold.org/cgi-bin/view.cgi?26Apr21-06-45-06a
The results will be erased in 6 hours..