Job Parameters

Submitted at Sun 01 Mar 2026 02:54:05 AM UTC

5'-CCATTGTGGTTGTTGCCGACTA-3' vs. 5'-UAAUUUCUACUAAGUGUAGAUCCAUUGUGGUUGUUGCCGACUA-3'
From 0°C by 2.5°C to 50°C as DNA
[A₀] = 0.00001 M, [B₀] = 0.00001 M
[Na⁺] = 1 M, [Mg⁺⁺] = 0 M (oligo mode)

Computation took 1.0 seconds (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
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Heat Capacity Plot	Absorbance Plot
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Download detailed plot: Postscript PDF	Download detailed plot: Postscript PDF

Probability Dot Plots

plot of A B A-A B-B A-B at 0 2.5 5 7.5 10 12.5 15 17.5 20 22.5 25 27.5 30 32.5 35 37.5 40 42.5 45 47.5 50 °C

Van't Hoff Plots

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Isothermal Titration Calorimetry Plots

Vary concentration of AB
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Important Numbers

ΔG: 17.7 kcal/mol   ΔH: 56.2 kcal/mol   ΔS: 185.4 cal/mol/K

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