The DINAMelt Server - results for 3

Job Parameters

Submitted at Sun 01 Mar 2026 03:00:11 AM UTC

5'-GGAAACAGTTTTTTTTTTTTTTTGCTGTTTCCTTCGAGCACC-3' vs. 5'-GGTGCTCGAGAGTGATTTTTTTTTTTTTTTATACCAGCTTATTCAATT-3'
From 37°C by 1°C to 100°C as DNA
[A0] = 1e-06 M, [B0] = 1e-06 M
[Na+] = 1 M, [Mg++] = 0 M (oligo mode)

Computation took 3.0 seconds (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
Concentration plot
Download: Postscript PDF Text
Heat Capacity Plot Absorbance Plot
Heat capacity plot Extinction plot
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Probability Dot Plots

°C

Van't Hoff Plots

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Isothermal Titration Calorimetry Plots


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Important Numbers

Tm(Conc): 75.5°C
ΔG: 10.1 kcal/mol   ΔH: 111.2 kcal/mol   ΔS: 328.4 cal/mol/K   Tm(Cp): 65.2°C
Tm(Ext1): 66.6°C   Tm(Ext2): 67.3°C

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