Job Parameters

Submitted at Sun 01 Mar 2026 05:34:19 AM UTC

5'-TGAAGAGAACACTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAATGTTTAGTTGAATCGTAGGAGGGAG-3' vs. 5'-TTTTTTTTTTTTTTTATCATCAATACTTC-3'
From 0°C by 5°C to 100°C as DNA
[A₀] = 0.00001 M, [B₀] = 0.00001 M
[Na⁺] = 1 M, [Mg⁺⁺] = 0 M (oligo mode)

Computation took 2.0 seconds (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
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Heat Capacity Plot	Absorbance Plot
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Probability Dot Plots

plot of A B A-A B-B A-B at 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 °C

Van't Hoff Plots

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Isothermal Titration Calorimetry Plots

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Important Numbers

T_m(Conc): 63.9°C
ΔG: 15.7 kcal/mol   ΔH: 98.6 kcal/mol   ΔS: 306.1 cal/mol/K   T_m(C_p): 73.4°C
T_m(Ext1): 76.5°C   T_m(Ext2): 46.7°C

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