Job Parameters

Submitted at Mon 20 Apr 2026 02:12:16 AM UTC

5'-AGTATGACTGATTTACGAACATTTCGGGGGACTGCTCGGGATTGCGGATACGATGTGTT-3' vs. 5'-CGTAAATCAGTCATACTGGGCGCGTAGATGACGAGCAGTCCTAACATCGTTTAGGAC-3'
From 36°C by 0.5°C to 46°C as DNA
[A₀] = 1e-05 M, [B₀] = 1e-05 M
[Na⁺] = 0.15 M, [Mg⁺⁺] = 0.005 M (oligo mode)

Computation took 14.0 seconds (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
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Heat Capacity Plot	Absorbance Plot
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Probability Dot Plots

plot of A B A-A B-B A-B at 36 36.5 37 37.5 38 38.5 39 39.5 40 40.5 41 41.5 42 42.5 43 43.5 44 44.5 45 45.5 46 °C

Van't Hoff Plots

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Isothermal Titration Calorimetry Plots

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Important Numbers

ΔG: 6.1 kcal/mol   ΔH: 19.5 kcal/mol   ΔS: 61.9 cal/mol/K

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