The DINAMelt Server - results for unnamed job 260420_044754

Job Parameters

Submitted at Mon 20 Apr 2026 04:48:20 AM UTC

5'-CAAGACTGGAAGACCC-3' vs. 5'-CAAGACGGGAAGACCC-3'
From 0°C by 1°C to 95°C as DNA
[A0] = 5e-12 M, [B0] = 5e-08 M
[Na+] = 0.05 M, [Mg++] = 0.003 M (oligo mode)

Computation took 11.0 seconds (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
Concentration plot
Download: Postscript PDF Text
Heat Capacity Plot Absorbance Plot
Heat capacity plot Extinction plot
Download: Postscript PDF Text Download: Postscript PDF Text
Download detailed plot: Postscript PDF Download detailed plot: Postscript PDF

Probability Dot Plots

°C

Important Numbers

Tm(Conc): 55.8°C
ΔG: 5.0 kcal/mol   ΔH: 28.6 kcal/mol   ΔS: 88.2 cal/mol/K   Tm(Cp): 53.6°C
Tm(Ext1): 53.1°C   Tm(Ext2): 52.6°C

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