Job Parameters

Submitted at Tue 21 Apr 2026 01:58:55 AM UTC

5'-CCCCACCTCCTCGCGCGTCTACACATGGCGCGCGAGGAGGT-3' vs. 5'-CCTCGCGCGTCTACACATGGCGCGCGAGGAGGTCCATGTGTAGA-3'
From 32°C by 0.5°C to 42°C as DNA
[A₀] = 0.00001 M, [B₀] = 0.00001 M
[Na⁺] = 1 M, [Mg⁺⁺] = 0 M (oligo mode)

Computation took 35.0 seconds (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
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Heat Capacity Plot	Absorbance Plot
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Download detailed plot: Postscript PDF	Download detailed plot: Postscript PDF

Probability Dot Plots

plot of A B A-A B-B A-B at 32 32.5 33 33.5 34 34.5 35 35.5 36 36.5 37 37.5 38 38.5 39 39.5 40 40.5 41 41.5 42 °C

Van't Hoff Plots

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Isothermal Titration Calorimetry Plots

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Important Numbers

ΔG: 6.4 kcal/mol   ΔH: 1.5 kcal/mol   ΔS: 4.8 cal/mol/K   T_m(C_p): 40.9°C
T_m(Ext1): 35.8°C

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