The DINAMelt Server - results for IL-6 and cortisol

Job Parameters

Submitted at Tue 21 Apr 2026 02:01:29 AM UTC

5'-GGTGGCAGGAGGACTATTTATTTGCTTTTCT-3' vs. 5'-CTCTCGGGACGACGCCAGAAGTTTACGAGGATATGGTAACATAGTCGTCCCTTTTTTTTTTTTGGTCTTCAAATGCT-3'
From 0°C by 1°C to 95°C as RNA
[A0] = 1e-05 M, [B0] = 1e-05 M
[Na+] = 1 M, [Mg++] = 0 M (oligo mode)

Computation took 1:50 (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
Concentration plot
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Heat Capacity Plot Absorbance Plot
Heat capacity plot Extinction plot
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Probability Dot Plots

°C

Van't Hoff Plots

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Isothermal Titration Calorimetry Plots


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Important Numbers

Tm(Conc): 82.0°C
ΔG: 57.6 kcal/mol   ΔH: 316.2 kcal/mol   ΔS: 950.2 cal/mol/K   Tm(Cp): 84.2°C
Tm(Ext1): 84.3°C   Tm(Ext2): 70.7°C

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