The DINAMelt Server - results for unnamed job 260421_020328

Job Parameters

Submitted at Tue 21 Apr 2026 02:05:25 AM UTC

5'-GATCAGTCTAGTTCA-3' vs. 5'-TCACTTTCATAATGCTGG-3'
From 0°C by 1°C to 100°C as DNA
[A0] = 1e-08 M, [B0] = 1e-08 M
[Na+] = 0.14 M, [Mg++] = 0.01 M (oligo mode)

Computation took 1:43 (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
Concentration plot
Download: Postscript PDF Text
Heat Capacity Plot Absorbance Plot
Heat capacity plot Extinction plot
Download: Postscript PDF Text Download: Postscript PDF Text
Download detailed plot: Postscript PDF Download detailed plot: Postscript PDF

Probability Dot Plots

°C

Van't Hoff Plots

View a van't Hoff plot as...
The fist time you request a plot there will be a short delay while the van't Hoff data is calculated.

Isothermal Titration Calorimetry Plots


:
View ITC plot as...
There will be a short delay while the ITC data is calculated.

Important Numbers

Tm(Conc): 24.7°C
ΔG: 4.6 kcal/mol   ΔH: 42.2 kcal/mol   ΔS: 140.2 cal/mol/K   Tm(Cp): 17.6°C
Tm(Ext1): 17.2°C   Tm(Ext2): 21.6°C

Download the entire job with all files, formatted as: tar/bzip2 tar/gzip zip
The first time you ask for an archive there may be a slight delay while it is created.