Job Parameters

Submitted at Tue 21 Apr 2026 02:40:43 AM UTC

5'-CCCCACCTCCTCGCGCGTCTACACATGGCGCGCGAGGAGGT-3' vs. 5'-CGGGCGUGGUGGUGGGGG-3'
From 32°C by 0.5°C to 42°C as DNA
[A₀] = 0.00001 M, [B₀] = 0.00001 M
[Na⁺] = 1 M, [Mg⁺⁺] = 0 M (oligo mode)

Computation took 7.0 seconds (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
Download: Postscript PDF Text
Heat Capacity Plot	Absorbance Plot
Download: Postscript PDF Text	Download: Postscript PDF Text
Download detailed plot: Postscript PDF	Download detailed plot: Postscript PDF

Probability Dot Plots

plot of A B A-A B-B A-B at 32 32.5 33 33.5 34 34.5 35 35.5 36 36.5 37 37.5 38 38.5 39 39.5 40 40.5 41 41.5 42 °C

Van't Hoff Plots

View a van't Hoff plot as...
The fist time you request a plot there will be a short delay while the van't Hoff data is calculated.

Isothermal Titration Calorimetry Plots

Vary concentration of AB
Enter one or more temperatures, separated by commas:
View ITC plot as...
There will be a short delay while the ITC data is calculated.

Important Numbers

ΔG: 3.7 kcal/mol   ΔH: 2.4 kcal/mol   ΔS: 7.7 cal/mol/K   T_m(C_p): 32.2°C
T_m(Ext1): 32.2°C

Download the entire job with all files, formatted as: tar/bzip2 tar/gzip zip
The first time you ask for an archive there may be a slight delay while it is created.