Job Parameters

Submitted at Tue 21 Apr 2026 02:44:17 AM UTC

5'-CCCTAACCCTAACCCTAACCCCTTTACCTGGGGGAGTATTGCGGAGGAAGGT-3' vs. 5'-GGGTTAGGGTTTCCCTAACCCTAACCCTTTACCTGGGGGAGTTCAGCATACGACACTCCCCCAGGTAAA-3'
From 31°C by 0.55°C to 42°C as DNA
[A₀] = 0.00001 M, [B₀] = 0.00001 M
[Na⁺] = 1 M, [Mg⁺⁺] = 0 M (oligo mode)

Computation took 26.0 seconds (Log file)

Warning: no significant hybridization of your sequences was found.
This may indicate an error in the sequences and/or strand concentrations.

Plots

Concentration Plot
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Heat Capacity Plot	Absorbance Plot
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Probability Dot Plots

plot of A B A-A B-B A-B at 31 31.55 32.1 32.65 33.2 33.75 34.3 34.85 35.4 35.95 36.5 37.05 37.6 38.15 38.7 39.25 39.8 40.35 40.9 41.45 42 °C

Van't Hoff Plots

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Isothermal Titration Calorimetry Plots

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Important Numbers

ΔG: 9.0 kcal/mol   ΔH: 23.2 kcal/mol   ΔS: 75.1 cal/mol/K   T_m(C_p): 31.8°C
T_m(Ext1): 32.0°C

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