The DINAMelt Server - results for A+B

Job Parameters

Submitted at Tue 21 Apr 2026 09:52:10 AM UTC

5'-ACATTCCTAAGTCTGAAACATTACAGCTTGCTACACGAGAAGAGCCGCCATAGTA-3' vs. 5'-TATCACCAGGCAGTTGACAGTGTAGCAAGCTGTAATAGATGCGAGGGTCCAATAC-3'
From 0°C by 1°C to 100°C as DNA
[A0] = 0.00001 M, [B0] = 0.00001 M
[Na+] = 0.1 M, [Mg++] = 0.01 M (oligo mode)

Computation took 33.0 seconds (Log file)

Plots

Concentration Plot
Concentration plot
Download: Postscript PDF Text
Heat Capacity Plot Absorbance Plot
Heat capacity plot Extinction plot
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Probability Dot Plots

°C

Van't Hoff Plots

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Isothermal Titration Calorimetry Plots


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Important Numbers

Tm(Conc): 71.3°C
ΔG: 42.1 kcal/mol   ΔH: 251.4 kcal/mol   ΔS: 768.2 cal/mol/K   Tm(Cp): 63.9°C
Tm(Ext1): 61.6°C   Tm(Ext2): 55.7°C

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