The DINAMelt Server - free energy decomposition results for unnamed job 260420_041906
Loop Free-Energy Decomposition
ΔG = -5.3 kcal/mol ΔH = -96.8 kcal/mol ΔS = -306.8 e.u. Tm = 10.3°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | +0.10 | 13 ss bases & 1 closing helices |
| Stack | -1.14 | External closing pair is T14-A35 |
| Stack | -1.44 | External closing pair is T15-A34 |
| Stack | -2.36 | External closing pair is C16-G33 |
| Helix | -4.93 | 4 base pairs |
| Bulge loop | +3.24 | External closing pair is G17-C32 |
| Stack | -1.58 | External closing pair is G21-C31 |
| Stack | -1.14 | External closing pair is T22-A30 |
| Helix | -2.71 | 3 base pairs |
| Interior loop | +2.65 | External closing pair is T23-A29 |
| Stack | -1.14 | External closing pair is T25-A26 |
| Stack | -1.59 | External closing pair is T26-A25 |
| Helix | -2.73 | 3 base pairs |
| Interior loop | +2.02 | External closing pair is G27-C24 |
| Stack | -1.14 | External closing pair is A29-T21 |
| Stack | -0.99 | External closing pair is A30-T20 |
| Stack | -1.59 | External closing pair is T31-A19 |
| Helix | -3.72 | 4 base pairs |
| External loop | +0.74 | Closing pair is G32-C18 |
ΔG = -4.6 kcal/mol ΔH = -82.4 kcal/mol ΔS = -260.9 e.u. Tm = 5.5°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.17 | 24 ss bases & 1 closing helices |
| Stack | -0.99 | External closing pair is A19-T29 |
| Stack | -1.59 | External closing pair is T20-A28 |
| Helix | -2.58 | 3 base pairs |
| Interior loop | +2.27 | External closing pair is G21-C27 |
| Stack | -1.58 | External closing pair is G24-C23 |
| Helix | -1.58 | 2 base pairs |
| Interior loop | +2.51 | External closing pair is T25-A22 |
| Stack | -0.70 | External closing pair is T28-A20 |
| Stack | -1.14 | External closing pair is A29-T19 |
| Stack | -0.99 | External closing pair is A30-T18 |
| Stack | +0.54 | External closing pair is T31-A17 |
| Stack | -0.28 | External closing pair is G32-T16 |
| Stack | -2.40 | External closing pair is G33-C15 |
| Helix | -4.97 | 7 base pairs |
| External loop | +0.92 | Closing pair is C34-G14 |
ΔG = -3.1 kcal/mol ΔH = -29.9 kcal/mol ΔS = -89.9 e.u. Tm = -33.4°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.04 | 36 ss bases & 1 closing helices |
| Stack | -1.58 | External closing pair is G21-C19 |
| Stack | -1.14 | External closing pair is T22-A18 |
| Helix | -2.71 | 3 base pairs |
| External loop | +0.66 | Closing pair is T23-A17 |
ΔG = -5.1 kcal/mol ΔH = -41.8 kcal/mol ΔS = -123.1 e.u. Tm = -8.4°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.66 | 32 ss bases & 1 closing helices |
| Stack | -1.58 | External closing pair is G27-C29 |
| Stack | -0.70 | External closing pair is T28-A28 |
| Stack | -1.14 | External closing pair is A29-T27 |
| Stack | -0.99 | External closing pair is A30-T26 |
| Helix | -4.41 | 5 base pairs |
| External loop | +0.98 | Closing pair is T31-A25 |
ΔG = -7.5 kcal/mol ΔH = -77.6 kcal/mol ΔS = -235.2 e.u. Tm = 14.3°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.35 | 32 ss bases & 1 closing helices |
| Stack | -1.58 | External closing pair is G21-C23 |
| Stack | -1.14 | External closing pair is T22-A22 |
| Stack | -1.59 | External closing pair is T23-A21 |
| Stack | -1.58 | External closing pair is G24-C20 |
| Helix | -5.88 | 5 base pairs |
| Interior loop | +1.59 | External closing pair is T25-A19 |
| Stack | -0.61 | External closing pair is G27-C17 |
| Helix | -0.61 | 2 base pairs |
| Interior loop | +2.18 | External closing pair is T28-G16 |
| Stack | -0.99 | External closing pair is A30-T13 |
| Stack | -1.59 | External closing pair is T31-A12 |
| Stack | -1.95 | External closing pair is G32-C11 |
| Helix | -4.53 | 4 base pairs |
| External loop | +1.12 | Closing pair is G33-C10 |
ΔG = -3.2 kcal/mol ΔH = -47.1 kcal/mol ΔS = -147.3 e.u. Tm = -14.5°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.35 | 30 ss bases & 1 closing helices |
| Stack | -1.58 | External closing pair is G24-C28 |
| Stack | +0.41 | External closing pair is T25-A27 |
| Stack | -0.48 | External closing pair is T26-G26 |
| Helix | -1.65 | 4 base pairs |
| Interior loop | +1.27 | External closing pair is G27-C25 |
| Stack | -1.14 | External closing pair is A29-T23 |
| Stack | -0.99 | External closing pair is A30-T22 |
| Helix | -2.13 | 3 base pairs |
| External loop | +0.66 | Closing pair is T31-A21 |
ΔG = -4.2 kcal/mol ΔH = -32.0 kcal/mol ΔS = -93.2 e.u. Tm = -23.2°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -0.78 | 35 ss bases & 1 closing helices |
| Stack | -1.95 | External closing pair is G32-C31 |
| Stack | -2.40 | External closing pair is G33-C30 |
| Helix | -4.35 | 3 base pairs |
| External loop | +0.92 | Closing pair is C34-G29 |
ΔG = -3.9 kcal/mol ΔH = -37.0 kcal/mol ΔS = -111.2 e.u. Tm = -19.6°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.50 | 21 ss bases & 1 closing helices |
| Stack | -1.40 | External closing pair is C12-G25 |
| Helix | -1.40 | 2 base pairs |
| Interior loop | +1.70 | External closing pair is T13-A24 |
| Stack | -2.36 | External closing pair is C16-G21 |
| Stack | -1.58 | External closing pair is G17-C20 |
| Helix | -3.94 | 3 base pairs |
| External loop | +1.29 | Closing pair is T18-A19 |
ΔG = -5.4 kcal/mol ΔH = -35.4 kcal/mol ΔS = -100.6 e.u. Tm = -6.2°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -0.71 | 31 ss bases & 1 closing helices |
| Stack | -1.95 | External closing pair is G32-C35 |
| Stack | -2.40 | External closing pair is G33-C34 |
| Stack | -1.95 | External closing pair is C34-G33 |
| Helix | -6.29 | 4 base pairs |
| External loop | +1.59 | Closing pair is C35-G32 |
ΔG = -3.0 kcal/mol ΔH = -49.0 kcal/mol ΔS = -154.4 e.u. Tm = -14.2°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.04 | 29 ss bases & 1 closing helices |
| Stack | -1.40 | External closing pair is C12-G17 |
| Stack | -1.14 | External closing pair is T13-A16 |
| Helix | -2.54 | 3 base pairs |
| Interior loop | +2.14 | External closing pair is T14-A15 |
| Stack | -2.36 | External closing pair is C16-G12 |
| Helix | -2.36 | 2 base pairs |
| External loop | +0.85 | Closing pair is G17-C11 |
ΔG = -3.7 kcal/mol ΔH = -25.7 kcal/mol ΔS = -73.8 e.u. Tm = -36.5°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -0.28 | 51 ss bases & 1 closing helices |
| Stack | -0.99 | External closing pair is A30-T13 |
| Stack | -1.59 | External closing pair is T31-A12 |
| Stack | -1.95 | External closing pair is G32-C11 |
| Helix | -4.53 | 4 base pairs |
| External loop | +1.12 | Closing pair is G33-C10 |
ΔG = -3.7 kcal/mol ΔH = -25.7 kcal/mol ΔS = -73.8 e.u. Tm = -36.5°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -0.28 | 36 ss bases & 1 closing helices |
| Stack | -0.99 | External closing pair is A30-T28 |
| Stack | -1.59 | External closing pair is T31-A27 |
| Stack | -1.95 | External closing pair is G32-C26 |
| Helix | -4.53 | 4 base pairs |
| External loop | +1.12 | Closing pair is G33-C25 |
ΔG = -3.5 kcal/mol ΔH = -41.1 kcal/mol ΔS = -126.3 e.u. Tm = -17.9°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.23 | 26 ss bases & 1 closing helices |
| Stack | -0.99 | External closing pair is A19-T27 |
| Stack | +0.54 | External closing pair is T20-A26 |
| Stack | +0.10 | External closing pair is G21-T25 |
| Stack | -1.14 | External closing pair is T22-A24 |
| Stack | -1.59 | External closing pair is T23-A23 |
| Helix | -3.07 | 6 base pairs |
| External loop | +0.85 | Closing pair is G24-C22 |
ΔG = -6.5 kcal/mol ΔH = -48.5 kcal/mol ΔS = -140.8 e.u. Tm = 3.0°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.35 | 31 ss bases & 1 closing helices |
| Stack | -1.58 | External closing pair is G24-C27 |
| Stack | -1.14 | External closing pair is T25-A26 |
| Stack | -1.59 | External closing pair is T26-A25 |
| Stack | -1.58 | External closing pair is G27-C24 |
| Helix | -5.88 | 5 base pairs |
| External loop | +0.72 | Closing pair is T28-A23 |
ΔG = -4.2 kcal/mol ΔH = -27.3 kcal/mol ΔS = -77.6 e.u. Tm = -30.2°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -0.76 | 50 ss bases & 1 closing helices |
| Stack | -0.99 | External closing pair is A30-T14 |
| Stack | -1.59 | External closing pair is T31-A13 |
| Stack | -1.95 | External closing pair is G32-C12 |
| Helix | -4.53 | 4 base pairs |
| External loop | +1.12 | Closing pair is G33-C11 |
ΔG = -3.6 kcal/mol ΔH = -36.9 kcal/mol ΔS = -111.8 e.u. Tm = -21.4°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.04 | 27 ss bases & 1 closing helices |
| Stack | -1.40 | External closing pair is C12-G19 |
| Stack | -1.14 | External closing pair is T13-A18 |
| Stack | -1.14 | External closing pair is T14-A17 |
| Helix | -3.67 | 4 base pairs |
| External loop | +1.14 | Closing pair is T15-A16 |
ΔG = -3.7 kcal/mol ΔH = -38.1 kcal/mol ΔS = -115.5 e.u. Tm = -19.7°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.05 | 30 ss bases & 1 closing helices |
| Stack | -1.58 | External closing pair is G17-C21 |
| Stack | -0.70 | External closing pair is T18-A20 |
| Stack | -0.99 | External closing pair is A19-T19 |
| Helix | -3.27 | 4 base pairs |
| External loop | +0.66 | Closing pair is T20-A18 |
ΔG = -7.2 kcal/mol ΔH = -87.4 kcal/mol ΔS = -269.1 e.u. Tm = 14.5°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.55 | 22 ss bases & 1 closing helices |
| Stack | -2.36 | External closing pair is C16-G28 |
| Helix | -2.36 | 2 base pairs |
| Interior loop | +1.61 | External closing pair is G17-C27 |
| Stack | +0.54 | External closing pair is T20-A24 |
| Stack | +0.10 | External closing pair is G21-T23 |
| Stack | -1.14 | External closing pair is T22-A22 |
| Stack | -1.59 | External closing pair is T23-A21 |
| Helix | -2.08 | 5 base pairs |
| Interior loop | +1.17 | External closing pair is G24-C20 |
| Stack | -1.59 | External closing pair is T26-A18 |
| Stack | -0.61 | External closing pair is G27-C17 |
| Helix | -2.20 | 3 base pairs |
| Bulge loop | +3.25 | External closing pair is T28-G16 |
| Stack | -1.59 | External closing pair is T31-A15 |
| Stack | -1.95 | External closing pair is G32-C14 |
| Stack | -2.40 | External closing pair is G33-C13 |
| Helix | -5.94 | 4 base pairs |
| External loop | +0.92 | Closing pair is C34-G12 |
ΔG = -5.7 kcal/mol ΔH = -60.9 kcal/mol ΔS = -185.1 e.u. Tm = 3.8°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.04 | 26 ss bases & 1 closing helices |
| Stack | -1.95 | External closing pair is C11-G19 |
| Stack | -1.40 | External closing pair is C12-G18 |
| Helix | -3.35 | 3 base pairs |
| Interior loop | +1.64 | External closing pair is T13-A17 |
| Stack | -2.36 | External closing pair is C16-G15 |
| Stack | -1.58 | External closing pair is G17-C14 |
| Helix | -3.94 | 3 base pairs |
| Interior loop | +1.74 | External closing pair is T18-A13 |
| Stack | -1.59 | External closing pair is T20-A11 |
| Helix | -1.59 | 2 base pairs |
| External loop | +0.85 | Closing pair is G21-C10 |
ΔG = -5.8 kcal/mol ΔH = -42.8 kcal/mol ΔS = -124.2 e.u. Tm = -4.0°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.17 | 12 ss bases & 1 closing helices |
| Stack | -0.99 | External closing pair is A3-T25 |
| Stack | -1.14 | External closing pair is T4-A24 |
| Stack | -1.44 | External closing pair is T5-A23 |
| Stack | -1.95 | External closing pair is C6-G22 |
| Helix | -5.51 | 5 base pairs |
| External loop | +0.92 | Closing pair is C7-G21 |
ΔG = -3.4 kcal/mol ΔH = -43.0 kcal/mol ΔS = -132.8 e.u. Tm = -16.6°C
| Structural element | δδG | Information |
|---|---|---|
| External loop | -1.35 | 35 ss bases & 1 closing helices |
| Stack | -1.58 | External closing pair is G27-C26 |
| Helix | -1.58 | 2 base pairs |
| Interior loop | +1.97 | External closing pair is T28-A25 |
| Stack | -1.59 | External closing pair is T31-A22 |
| Stack | -1.95 | External closing pair is G32-C21 |
| Helix | -3.54 | 3 base pairs |
| External loop | +1.08 | Closing pair is G33-C20 |