Loop Free-Energy Decomposition

Dimer 1

ΔG = -6.4 kcal/mol ΔH = -57.0 kcal/mol ΔS = -169.6 e.u. T_m = 9.5°C

Structural element	δδG	Information
External loop	-1.59	29 ss bases & 1 closing helices
Stack	-1.44	External closing pair is G22-C27
Stack	-0.99	External closing pair is A23-T26
Stack	-0.70	External closing pair is T24-A25
Stack	-0.99	External closing pair is A25-T24
Stack	-1.44	External closing pair is T26-A23
Helix	-5.56	6 base pairs
External loop	+0.72	Closing pair is C27-G22

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Dimer 2

ΔG = -5.6 kcal/mol ΔH = -39.2 kcal/mol ΔS = -112.7 e.u. T_m = -7.4°C

Structural element	δδG	Information
External loop	-0.86	49 ss bases & 1 closing helices
Stack	-1.14	External closing pair is A30-T15
Stack	-1.58	External closing pair is A31-T14
Stack	-1.59	External closing pair is C32-G13
Stack	-1.58	External closing pair is A33-T12
Helix	-5.88	5 base pairs
External loop	+1.14	Closing pair is C34-G11

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Dimer 3

ΔG = -3.3 kcal/mol ΔH = -26.5 kcal/mol ΔS = -77.7 e.u. T_m = -37.5°C

Structural element	δδG	Information
External loop	-0.86	47 ss bases & 1 closing helices
Stack	-1.14	External closing pair is A30-T17
Stack	-1.58	External closing pair is A31-T16
Helix	-2.71	3 base pairs
External loop	+0.23	Closing pair is C32-G15

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Dimer 4

ΔG = -2.9 kcal/mol ΔH = -30.5 kcal/mol ΔS = -92.5 e.u. T_m = -33.6°C

Structural element	δδG	Information
External loop	-1.04	19 ss bases & 1 closing helices
Stack	-1.58	External closing pair is G15-C30
Stack	-1.44	External closing pair is T16-A29
Helix	-3.01	3 base pairs
External loop	+1.14	Closing pair is C17-G28

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Dimer 5

ΔG = -4.7 kcal/mol ΔH = -35.2 kcal/mol ΔS = -102.4 e.u. T_m = -16.6°C

Structural element	δδG	Information
External loop	-1.50	25 ss bases & 1 closing helices
Stack	-1.40	External closing pair is C19-G28
Stack	-0.70	External closing pair is T20-A27
Stack	-1.40	External closing pair is A21-T26
Helix	-3.50	4 base pairs
External loop	+0.35	Closing pair is G22-C25

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Dimer 6

ΔG = -6.3 kcal/mol ΔH = -72.9 kcal/mol ΔS = -223.4 e.u. T_m = 9.2°C

Structural element	δδG	Information
External loop	-1.17	12 ss bases & 1 closing helices
Stack	-0.99	External closing pair is A3-T25
Stack	-1.14	External closing pair is T4-A24
Stack	-1.44	External closing pair is T5-A23
Stack	-1.95	External closing pair is C6-G22
Helix	-5.51	5 base pairs
Interior loop	+4.26	External closing pair is C7-G21
Stack	-1.95	External closing pair is C11-G14
Stack	-1.59	External closing pair is C12-G13
Stack	-0.99	External closing pair is A13-T12
Helix	-4.53	4 base pairs
External loop	+0.66	Closing pair is T14-A11

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Dimer 7

ΔG = -2.9 kcal/mol ΔH = -30.7 kcal/mol ΔS = -93.1 e.u. T_m = -33.1°C

Structural element	δδG	Information
External loop	-0.75	40 ss bases & 1 closing helices
Stack	-0.99	External closing pair is A25-T19
Stack	-1.44	External closing pair is T26-A18
Helix	-2.43	3 base pairs
External loop	+0.23	Closing pair is C27-G17