Loop Free-Energy Decomposition

Dimer 1

ΔG = -29.6 kcal/mol ΔH = -202.2 kcal/mol ΔS = -556.6 e.u. T_m = 71.4°C

Structural element	δδG	Information
External loop	-0.54	44 ss bases & 1 closing helices
Stack	-1.30	External closing pair is G45-C68
Stack	-0.86	External closing pair is T46-A67
Stack	-0.44	External closing pair is T47-A66
Stack	-1.14	External closing pair is A48-T65
Stack	-2.10	External closing pair is G49-C64
Stack	-1.14	External closing pair is C50-G63
Stack	-0.86	External closing pair is T51-A62
Stack	-1.31	External closing pair is T52-A61
Stack	-1.70	External closing pair is G53-C60
Stack	-1.70	External closing pair is G54-C59
Stack	-1.30	External closing pair is G55-C58
Stack	-0.86	External closing pair is T56-A57
Stack	-1.16	External closing pair is T57-A56
Stack	-2.03	External closing pair is C58-G55
Stack	-2.10	External closing pair is G59-C54
Stack	-1.70	External closing pair is C60-G53
Stack	-1.31	External closing pair is C61-G52
Stack	-0.86	External closing pair is A62-T51
Stack	-0.74	External closing pair is A63-T50
Stack	-1.16	External closing pair is T64-A49
Stack	-1.31	External closing pair is C65-G48
Stack	-1.30	External closing pair is A66-T47
Stack	-2.03	External closing pair is C67-G46
Helix	-30.47	24 base pairs
External loop	+1.45	Closing pair is G68-C45