Loop Free-Energy Decomposition

Dimer 1

ΔG = -27.9 kcal/mol ΔH = -187.5 kcal/mol ΔS = -514.7 e.u. T_m = 71.0°C

Structural element	δδG	Information
External loop	-1.36	12 ss bases & 1 closing helices
Stack	-1.31	External closing pair is C12-G33
Stack	-1.14	External closing pair is A13-T32
Stack	-2.10	External closing pair is G14-C31
Stack	-1.70	External closing pair is C15-G30
Stack	-1.14	External closing pair is C16-G29
Stack	-0.86	External closing pair is T17-A28
Stack	-1.16	External closing pair is T18-A27
Stack	-1.31	External closing pair is C19-G26
Stack	-1.14	External closing pair is A20-T25
Stack	-1.16	External closing pair is G21-C24
Stack	-0.74	External closing pair is A22-T23
Stack	-1.16	External closing pair is T23-A22
Stack	-1.14	External closing pair is C24-G21
Stack	-1.16	External closing pair is T25-A20
Stack	-2.03	External closing pair is C26-G19
Stack	-2.10	External closing pair is G27-C18
Stack	-1.31	External closing pair is C28-G17
Stack	-1.14	External closing pair is A29-T16
Stack	-1.30	External closing pair is G30-C15
Stack	-1.16	External closing pair is T31-A14
Stack	-1.31	External closing pair is C32-G13
Stack	-0.86	External closing pair is A33-T12
Helix	-28.49	23 base pairs
External loop	+1.98	Closing pair is A34-T11

---

Dimer 2

ΔG = -3.6 kcal/mol ΔH = -28.8 kcal/mol ΔS = -81.2 e.u. T_m = -8.0°C

Structural element	δδG	Information
External loop	-0.81	30 ss bases & 1 closing helices
Stack	-1.14	External closing pair is A14-T17
Stack	-2.10	External closing pair is G15-C16
Stack	-1.14	External closing pair is C16-G15
Helix	-4.39	4 base pairs
External loop	+1.57	Closing pair is T17-A14