Loop Free-Energy Decomposition

Dimer 1

ΔG = -22.0 kcal/mol ΔH = -272.0 kcal/mol ΔS = -805.9 e.u. T_m = 53.9°C

Structural element	δδG	Information
External loop	-0.32	38 ss bases & 1 closing helices
Stack	-0.93	External closing pair is C27-G49
Stack	-0.76	External closing pair is A28-T48
Stack	-0.92	External closing pair is G29-C47
Stack	-0.78	External closing pair is T30-A46
Stack	-0.76	External closing pair is C31-G45
Stack	-0.93	External closing pair is T32-A44
Stack	-0.78	External closing pair is G33-C43
Stack	-0.36	External closing pair is A34-T42
Stack	-0.06	External closing pair is T35-A41
Stack	-0.48	External closing pair is A36-T40
Stack	-0.76	External closing pair is A37-T39
Stack	-1.72	External closing pair is G38-C38
Stack	-0.76	External closing pair is C39-G37
Stack	-0.06	External closing pair is T40-A36
Stack	-0.92	External closing pair is A41-T35
Stack	-1.32	External closing pair is C42-G34
Stack	-0.93	External closing pair is C43-G33
Stack	-0.36	External closing pair is A44-T32
Stack	-0.93	External closing pair is T45-A31
Stack	-0.92	External closing pair is G46-C30
Stack	-0.93	External closing pair is T47-A29
Stack	-0.92	External closing pair is G48-C28
Stack	-0.06	External closing pair is T49-A27
Stack	-0.76	External closing pair is A50-T26
Stack	-0.78	External closing pair is G51-C25
Stack	-0.36	External closing pair is A52-T24
Stack	-0.06	External closing pair is T53-A23
Stack	-0.76	External closing pair is A54-T22
Stack	-1.72	External closing pair is G55-C21
Stack	-0.76	External closing pair is C56-G20
Stack	-0.48	External closing pair is T57-A19
Stack	-0.06	External closing pair is T58-A18
Stack	-0.36	External closing pair is A59-T17
Helix	-23.29	34 base pairs
External loop	+1.56	Closing pair is T60-A16