Loop Free-Energy Decomposition

Dimer 1

ΔG = -36.6 kcal/mol ΔH = -224.6 kcal/mol ΔS = -606.2 e.u. T_m = 82.3°C

Structural element	δδG	Information
External loop	+0.00	0 ss bases & 1 closing helices
Stack	-2.24	External closing pair is G1-C28
Stack	-1.45	External closing pair is C2-G27
Stack	-1.44	External closing pair is A3-T26
Stack	-2.17	External closing pair is C4-G25
Stack	-1.44	External closing pair is G5-C24
Stack	-1.45	External closing pair is T6-A23
Stack	-1.44	External closing pair is G7-C22
Stack	-1.00	External closing pair is T8-A21
Stack	-1.45	External closing pair is T9-A20
Stack	-1.44	External closing pair is G10-C19
Stack	-1.30	External closing pair is T11-A18
Stack	-1.28	External closing pair is C12-G17
Stack	-1.30	External closing pair is T13-A16
Stack	-1.28	External closing pair is C14-G15
Stack	-1.30	External closing pair is T15-A14
Stack	-1.28	External closing pair is C16-G13
Stack	-1.45	External closing pair is T17-A12
Stack	-1.44	External closing pair is G18-C11
Stack	-1.45	External closing pair is T19-A10
Stack	-1.44	External closing pair is G20-C9
Stack	-1.30	External closing pair is T21-A8
Stack	-1.28	External closing pair is C22-G7
Stack	-1.30	External closing pair is T23-A6
Stack	-2.17	External closing pair is C24-G5
Helix	-35.09	25 base pairs
Interior loop	-1.22	External closing pair is G25-C4
Stack	-2.24	External closing pair is G27-C2
Helix	-2.24	2 base pairs
External loop	+1.96	Closing pair is C28-G1