Loop Free-Energy Decomposition

Dimer 1

ΔG = -15.7 kcal/mol ΔH = -147.3 kcal/mol ΔS = -424.4 e.u. T_m = 54.1°C

Structural element	δδG	Information
External loop	+0.00	0 ss bases & 1 closing helices
Stack	-2.24	External closing pair is G1-C28
Stack	-1.45	External closing pair is C2-G27
Stack	-1.44	External closing pair is A3-T26
Helix	-5.13	4 base pairs
Interior loop	-0.24	External closing pair is C4-G25
Stack	-1.45	External closing pair is T6-A23
Helix	-1.45	2 base pairs
Interior loop	+1.14	External closing pair is G7-C22
Stack	-1.45	External closing pair is T9-A20
Stack	-1.44	External closing pair is G10-C19
Helix	-2.89	3 base pairs
Interior loop	+2.38	External closing pair is T11-A18
Stack	-1.30	External closing pair is T13-A16
Helix	-1.30	2 base pairs
Interior loop	+0.33	External closing pair is C14-G15
Stack	-1.28	External closing pair is C16-G13
Helix	-1.28	2 base pairs
Interior loop	+0.31	External closing pair is T17-A12
Stack	-1.45	External closing pair is T19-A10
Stack	-1.44	External closing pair is G20-C9
Stack	-1.30	External closing pair is T21-A8
Helix	-4.19	4 base pairs
Interior loop	+0.33	External closing pair is C22-G7
Stack	-2.17	External closing pair is C24-G5
Helix	-2.17	2 base pairs
Interior loop	-1.22	External closing pair is G25-C4
Stack	-2.24	External closing pair is G27-C2
Helix	-2.24	2 base pairs
External loop	+1.96	Closing pair is C28-G1