Loop Free-Energy Decomposition

Dimer 1

ΔG = -25.8 kcal/mol ΔH = -203.5 kcal/mol ΔS = -604.2 e.u. T_m = 49.9°C

Structural element	δδG	Information
External loop	-0.89	12 ss bases & 1 closing helices
Stack	-1.51	External closing pair is C13-G71
Stack	-0.82	External closing pair is T14-A70
Stack	-1.10	External closing pair is A15-T69
Stack	-0.82	External closing pair is T16-A68
Stack	-1.51	External closing pair is A17-T67
Stack	-1.69	External closing pair is G18-C66
Stack	-1.71	External closing pair is T19-A65
Stack	-1.55	External closing pair is G20-C64
Stack	-1.51	External closing pair is A21-T63
Stack	-1.69	External closing pair is G22-C62
Stack	-1.55	External closing pair is T23-A61
Stack	-2.50	External closing pair is C24-G60
Stack	-1.69	External closing pair is G25-C59
Stack	-0.82	External closing pair is T26-A58
Stack	-1.10	External closing pair is A27-T57
Stack	-1.25	External closing pair is T28-A56
Stack	-0.82	External closing pair is T29-A55
Helix	-23.65	18 base pairs
Bulge loop	+4.09	External closing pair is A30-T54
Stack	-1.55	External closing pair is G38-C53
Stack	-1.51	External closing pair is A39-T52
Stack	-2.05	External closing pair is G40-C51
Stack	-1.55	External closing pair is G41-C50
Helix	-6.67	5 base pairs
Interior loop	+4.10	External closing pair is A42-T49
Stack	-1.69	External closing pair is G51-C41
Stack	-1.71	External closing pair is T52-A40
Helix	-3.40	3 base pairs
External loop	+0.66	Closing pair is G53-C39