Loop Free-Energy Decomposition

Dimer 1

ΔG = -19.1 kcal/mol ΔH = -179.6 kcal/mol ΔS = -517.4 e.u. T_m = 57.6°C

Structural element	δδG	Information
External loop	+0.05	0 ss bases & 1 closing helices
Stack	-0.99	External closing pair is A1-U23
Stack	-1.00	External closing pair is C2-G22
Stack	-0.99	External closing pair is A3-U21
Stack	-1.39	External closing pair is C4-G20
Stack	-1.39	External closing pair is C5-G19
Stack	-1.39	External closing pair is C6-G18
Stack	-1.00	External closing pair is C7-G17
Stack	-0.55	External closing pair is A8-U16
Stack	-0.55	External closing pair is A9-U15
Stack	-0.55	External closing pair is A10-U14
Stack	-0.43	External closing pair is A11-U13
Stack	-0.85	External closing pair is T12-A12
Stack	-1.72	External closing pair is C13-G11
Stack	-0.85	External closing pair is G14-C10
Stack	-0.55	External closing pair is A15-U9
Stack	-0.83	External closing pair is A16-U8
Stack	-1.79	External closing pair is G17-C7
Stack	-1.00	External closing pair is C18-G6
Stack	-0.99	External closing pair is A19-U5
Stack	-0.83	External closing pair is C20-G4
Stack	-0.55	External closing pair is T21-A3
Stack	-0.85	External closing pair is T22-A2
Helix	-21.13	23 base pairs
External loop	+1.96	Closing pair is C23-G1