Loop Free-Energy Decomposition

Dimer 1

ΔG = -4.5 kcal/mol ΔH = -30.3 kcal/mol ΔS = -83.1 e.u. T_m = 5.5°C

Structural element	δδG	Information
External loop	-0.46	23 ss bases & 1 closing helices
Stack	-1.45	External closing pair is T7-A5
Stack	-2.24	External closing pair is G8-C4
Stack	-1.28	External closing pair is C9-G3
Helix	-4.97	4 base pairs
External loop	+0.91	Closing pair is T10-A2