Loop Free-Energy Decomposition

Dimer 1

ΔG = -3.7 kcal/mol ΔH = -31.4 kcal/mol ΔS = -89.2 e.u. T_m = 0.3°C

Structural element	δδG	Information
External loop	-0.81	25 ss bases & 1 closing helices
Stack	-2.17	External closing pair is C12-G6
Stack	-1.84	External closing pair is G13-C5
Helix	-4.01	3 base pairs
External loop	+1.09	Closing pair is G14-C4