Loop Free-Energy Decomposition

Dimer 1

ΔG = -3.5 kcal/mol ΔH = -33.1 kcal/mol ΔS = -95.5 e.u. T_m = 0.0°C

Structural element	δδG	Information
External loop	-1.19	25 ss bases & 1 closing helices
Stack	-2.17	External closing pair is C12-G6
Helix	-2.17	2 base pairs
Interior loop	+1.60	External closing pair is G13-C5
Stack	-2.24	External closing pair is G15-C3
Helix	-2.24	2 base pairs
External loop	+0.53	Closing pair is C16-G2