Loop Free-Energy Decomposition

Dimer 1

ΔG = -25.2 kcal/mol ΔH = -176.8 kcal/mol ΔS = -488.8 e.u. T_m = 70.5°C

Structural element	δδG	Information
External loop	-0.50	35 ss bases & 1 closing helices
Stack	-1.62	External closing pair is G36-C22
Stack	-1.08	External closing pair is G37-C21
Stack	-0.78	External closing pair is A38-U20
Stack	-0.78	External closing pair is A39-U19
Stack	-1.06	External closing pair is A40-U18
Stack	-2.02	External closing pair is G41-C17
Stack	-1.95	External closing pair is C42-G16
Stack	-2.02	External closing pair is G43-C15
Stack	-1.62	External closing pair is C44-G14
Stack	-1.62	External closing pair is C45-G13
Stack	-1.62	External closing pair is C46-G12
Stack	-1.62	External closing pair is C47-G11
Stack	-1.23	External closing pair is C48-G10
Stack	-0.66	External closing pair is A49-U9
Stack	-0.78	External closing pair is T50-A8
Stack	-0.78	External closing pair is T51-A7
Stack	-0.78	External closing pair is T52-A6
Stack	-1.23	External closing pair is T53-A5
Stack	-1.08	External closing pair is G54-C4
Stack	-1.06	External closing pair is A55-U3
Stack	-1.22	External closing pair is G56-C2
Helix	-26.69	22 base pairs
External loop	+2.01	Closing pair is T57-A1