Loop Free-Energy Decomposition

Dimer 1

ΔG = -24.7 kcal/mol ΔH = -171.9 kcal/mol ΔS = -474.7 e.u. T_m = 70.4°C

Structural element	δδG	Information
External loop	+0.00	0 ss bases & 1 closing helices
Stack	-1.62	External closing pair is G1-C22
Stack	-1.08	External closing pair is G2-C21
Stack	-0.78	External closing pair is A3-U20
Stack	-0.78	External closing pair is A4-U19
Stack	-1.06	External closing pair is A5-U18
Stack	-2.02	External closing pair is G6-C17
Stack	-1.95	External closing pair is C7-G16
Stack	-2.02	External closing pair is G8-C15
Stack	-1.62	External closing pair is C9-G14
Stack	-1.62	External closing pair is C10-G13
Stack	-1.62	External closing pair is C11-G12
Stack	-1.62	External closing pair is C12-G11
Stack	-1.23	External closing pair is C13-G10
Stack	-0.66	External closing pair is A14-U9
Stack	-0.78	External closing pair is T15-A8
Stack	-0.78	External closing pair is T16-A7
Stack	-0.78	External closing pair is T17-A6
Stack	-1.23	External closing pair is T18-A5
Stack	-1.08	External closing pair is G19-C4
Stack	-1.06	External closing pair is A20-U3
Stack	-1.22	External closing pair is G21-C2
Helix	-26.69	22 base pairs
External loop	+2.01	Closing pair is T22-A1