RNA Folding Form

M. Zuker
Mfold web server for nucleic acid folding and hybridization prediction.
Nucleic Acids Res. 31 (13), 3406-15, (2003)
[Abstract] [Full Text] [Supplementary Material] [Additional Information]

The folding temperature is fixed at 37°. You may still fold with the older version 2.3 RNA parameters, which allow the temperature to be varied.

DNA mfold server. Quikfold. Fold many short RNA or DNA sequences at once.

   

Enter constraint information in the box at the right. (optional) You may:

  1. force bases i,i+1,...,i+k-1 to be double stranded by entering:
    F   i   0   k on 1 line in the constraint box.
  2. force consecutive base pairs i.j,i+1.j-1, ...,i+k-1.j-k+1 by entering:
    F   i   j   k on 1 line in the constraint box.
  3. force bases i,i+1,...,i+k-1 to be single stranded by entering:
    P   i   0   k on 1 line in the constraint box.
  4. prohibit the consecutive base pairs i.j,i+1.j-1, ...,i+k-1.j-k+1 by entering:
    P   i   j   k on 1 line in the constraint box.
  5. prohibit bases i to j from pairing with bases k to l by entering:
    P   i-j   k-l on 1 line in the constraint box.

Folding temperature is fixed at 37°.

Ionic conditions: 1M NaCl, no divalent ions.

Your job can be processed while you wait (the default) or can be submitted for batch processing by pressing the button below. In this case, you will be notified at a later time that the job is finished. If you select a batch job, please make sure your E-mail address is correct in the window below.

Choose image width for png & jpg files:
Choose structure format:
Grid lines in energy dot plot:
Choose structure annotation:
Current limits: 2400 bases for an immediate job, 2400 for batch.